Autoantibodies

IC61000 5-panel
4-color immunofluorescence image by Shangli Lian

Dr. Chan’s laboratory has a long history of the identification and characterization of specific autoimmune target antigens in different systemic rheumatic diseases. His early work focused on the study of human autoantibodies and autoantigens in systemic lupus erythematosus (SLE) and Sjögren’s syndrome (SjS), including the characterization of SS-B/La and the initial identification and cloning of the Ro52/TRIM21 autoantigen (J Clin Invest. 1991; 87:68-76). His laboratory has been highly successful in using unique autoimmune antibodies as probes to identify and clone a number of interesting macromolecules:

  • the ribosomal RNA transcription factor NOR90/hUBF, localized to the nucleolar organizer region (NOR) on mitotic chromosomes (J Exp Med. 1991; 174:1239-44)
  • p80-coilin, the primary marker for the Cajal body (J Exp Med. 1991; 173:1407-19)
  • a coiled-coil-rich protein family of Golgi autoantigens known as golgins (J Exp Med. 1993; 178:49-62; Arthritis Rheum. 1997; 40:1693-1702; Autoimmun Rev. 2005; 4:35-41)
  • GW182, a marker protein for cytoplasmic foci GW Bodies, also known as mammalian processing bodies (Mol Biol Cell. 2002; 13:1338-51)
  • rods and rings (RRs), large filamentous or annular assemblies of IMPDH, CTPS, and other proteins under investigation (PLoS One. 2011; 6:e29690; Antivir Ther. 2012; 17:805-11)

Dr. Chan’s laboratory also actively participates in the International Consensus on ANA Patterns (ICAP) – www.ANApatterns.org.

Publications

2019

Damoiseaux J, Andrade LEC, Carballo OG, Conrad K, Francescantonio PLC, Fritzler MJ, Garcia de la Torre I, Herold M, Klotz W, Cruvinel WM, Mimori T, von Mühlen CA, Satoh M, and Chan EKL. Clinical relevance of HEp-2 indirect immunofluorescent patterns: the International Consensus on ANA Patterns (ICAP) perspective. Ann Rheum Dis. In press. Online Mar 12, 2019.

Keppeke GD, Calise SJ, Chan EKL, and Andrade LEC. Ribavirin induces widespread accumulation of IMP dehydrogenase into rods/rings structures in multiple major mouse organs. Antiviral Res. 162:130-135

2018

Chan EKL. Importance of Autoantibody Standardization. In Perspectives in Autoimmunity (published by Grifols, Barcelona, Spain), 1:3-11.

Chan EKL and Andrade LEC. Clarification Needed Regarding Anti-Topoisomerase I as a Biomarker for Non-Small Cell Lung Cancer. Ann Thorac Surg. 106:935.

Meroni PL, Chan EKL, Damoiseaux J, Andrade LEC, Bossuyt X, Conrad K, Mariette X, Sheldon J, Rönnelid J, and Fritzler MJ. Unending story of the indirect immunofluorescence assay on HEp-2 cells: old problems and new solutions? Ann Rheum Dis. [Epub ahead of print].

Herold M, Klotz W, Andrade LEC, Conrad K, Cruvinel WM, Damoiseaux J, Fritzler MJ, von Mühlen CA, Satoh M, and Chan EKL. International Consensus on Antinuclear Antibody Patterns: defining negative results and reporting unidentified patterns. Clin Chem Lab Med. 56(10):1799-1802.

Calise SJ, Zheng B, Hasegawa T, Satoh M, Isailovic N, Ceribelli A, Andrade LEC, Boylan K, Cavazzana I, Fritzler MJ, Garcia de la Torre I, Hiepe F, Kohl K, Selmi C, Shoenfeld Y, Tincani A, Chan EKL. Reference standards for the detection of anti-mitochondrial and anti-rods/rings autoantibodies. Clin Chem Lab Med. 56(10):1789-1798.

Andrade LEC, Klotz W, Herold M, Conrad K, Rönnelid J, Fritzler MJ, von Mühlen CA, Satoh M, Damoiseaux J, Cruvinel WM, and Chan EKL; the Executive Committee of ICAP. International consensus on antinuclear antibody patterns: definition of the AC-29 pattern associated with antibodies to DNA topoisomerase I. Clin Chem Lab Med. 56(10):1783-1788.

Dinse GE, Parks CG, Meier HCS, Co CA, Chan EKL, Jusko TA, Yeh J, and Miller FW. Prescription medication use and antinuclear antibodies in the United States, 1999-2004. J Autoimmun. 92:93-103.

2017

Calise SJ and Chan EK. Advances in the immunobiology of autoantigenic rods/rings structures. In Immunodeficiency, Infection and Autoimmune Diseases – Report on the 13th Dresden Symposium on Autoantibodies (Conrad K, Andrade LEC, Chan EKL, Fritzler MJ, Pruijn GJM, Shoenfeld Y, and Steiner G, Eds.), Pabst Science Publishers, Lengerich, Germany, 2017.

Dinse GE, Parks CG, Weinberg CR, Meier HCS, Co CA, Chan EKL, and Miller FW. Antinuclear antibodies and mortality in the National Health and Nutrition Examination Survey (1999-2004). PLoS One. 12(10):e0185977.

Chan EK, Toubi E, and Conrad K. Biomarkers and Pathogenic Mechanisms in Autoimmunity. Immunol Res. 65(1):296-298.

Satoh, M., Tanaka, S., Ceribelli, A., Calise, S.J., and Chan, E.K. A Comprehensive Overview on Myositis-Specific Antibodies: New and Old Biomarkers in Idiopathic Inflammatory Myopathy. Clin Rev Allergy Immunol. 52(1):1-19.

2016

Calise, S.J., Bizzaro, N., Nguyen, T., Bassetti, D., Porcelli, B., Almi, P., Barberio, G., Pesce, G., Satoh, M., and Chan, E.K. Anti-rods/rings autoantibody seropositivity does not affect response to telaprevir treatment for chronic hepatitis C infection. Autoimmun Highlights. 7(1):15.

Uomori K, Nozawa K, Ikeda K, Doe K, Yamada Y, Yamaguchi A, Fujishiro M, Kawasaki M, Morimoto S, Takamori K, Sekigawa I, Chan EKL, and Takasaki Y. A re-evaluation of anti-NA-14 antibodies in patients with primary Sjögren’s syndrome: Significant role of interferon-γ in the production of autoantibodies against NA-14. Autoimmunity 49(5):347-56.

Keppeke, G.D., Prado, M.S., Nunes, E., Perazzio, S.F., Rodrigues, S.H., Ferraz, M.L., Chan, E.K., and Andrade, L.E. Differential capacity of therapeutic drugs to induce Rods/Rings structures in vitro and in vivo and generation of anti-Rods/Rings autoantibodies. Clin Immunol. In press.

Esfandiary L, Gupta N, Voigt A, Wanchoo A, Chan EK, Sukumaran S, and Nguyen CQ. Single-cell antibody nanowells: a novel technology in detecting anti-SSA/Ro60- and anti-SSB/La autoantibody-producing cells in peripheral blood of rheumatic disease patients. Arthritis Res Ther. 18(1):107.

Dinse, G.E., Jusko, T.A., Whitt, I.Z., Co, C.A., Parks, C.G., Satoh, M., Chan, E.K., Rose, K.M., Walker, N.J., Birnbaum, L.S., Zeldin, D.C., Weinberg, C.R., and Miller, F.W. Associations between Selected Xenobiotics and Antinuclear Antibodies in the National Health and Nutrition Examination Survey, 1999-2004. Environ Health Perspect. 124(4):426-36.

Keppeke, G.D., Calise, S.J., Chan, E.K., and Andrade, L.E. Anti-rods/rings autoantibody generation in hepatitis C patients during interferon-α/ribavirin therapy. World J Gastroenterol. 22(6):1966-74. Review.

Fritzler, M.J. and Chan, E.K. Dr. Eng M. Tan: a tribute to an enduring legacy in autoimmunity. Lupus. Aug 17. pii: 0961203316664598. [Epub ahead of print]

Chan, E.K., Burlingame, R.W., and Fritzler, M.J. Detection of autoantibodies by enzyme-linked immunosorbent assay and bead assays. In Manual of Molecular and Clinical Laboratory Immunology, 8th ed. edited by Detrick, B., Schmitz, J.L., Hamilton, R.G., American Society for Microbiology Press, Washington, D.C., Chapter 88, p859-67.

Satoh, M, Ceribelli, A., Hirakata, M., and Chan, E.K. Immunodiagnosis of autoimmune myopathies. In Manual of Molecular and Clinical Laboratory Immunology, 8th ed. edited by Detrick, B., Schmitz, J.L., Hamilton, R.G., American Society for Microbiology Press, Washington, D.C., Chapter 90, p878-87.

Damoiseaux, J., von Mühlen, C.A., Garcia-De La Torre, I., Carballo, O.G., de Melo Cruvinel, W., Francescantonio, P.L., Fritzler, M.J., Herold, M., Mimori, T., Satoh, M., Andrade, L.E., Chan, E.K., and Conrad, K. International Consensus on ANA Patterns (ICAP): the bumpy road towards a consensus on reporting ANA results. Auto Immun Highlights. 7(1):1.

Satoh, M., Fritzler, M.J., and Chan, E.K. Anti-histone and anti-spliceosome antibodies. In Systemic Lupus Erythematosus – A Manual, edited by Tsokos, G., Academic Press/Elsevier, Chapter 25, p213-21.

Conrad, K., Andrade, L.E., Chan, E.K., Mahler, M., Meroni, P,L., Pruijn, G.J., Steiner, G., and Shoenfeld, Y. From autoantibody research to standardized diagnostic assays in the management of human diseases – Report of the 12 Dresden Symposium on Autoantibodies. Lupus. 25(8):787-96.

Chan, E.K., Damoiseaux, J., de Melo Cruvinel, W., Carballo, O.G., Conrad, K., Francescantonio, P.L., Fritzler, M.J., Garcia de la Torre, I., Herold, M., Mimori, T., Satoh, M., von Mühlen, C.A., and Andrade, L.E. Report on the second International Consensus on ANA Pattern (ICAP) workshop in Dresden 2015. Lupus. 25(8):797-804.

von Mühlen, C.A., Fritzler, M.J., and Chan, E.K. Clinical and laboratory evaluation of systemic rheumatic diseases. In Henry’s Clinical Diagnosis and Management by Laboratory Methods, edited by Richard McPherson and Matthew Pincus, 23rd edition. Chapter 52. Elsevier, p993-1015.

2015

Chan, E.K., Damoiseaux, J., Carballo, O.G., Conrad, K., de Melo Cruvinel, W., Francescantonio, P.L., Fritzler, M.J., Garcia-De La Torre, I., Herold, M., Mimori, T., Satoh, M., von Mühlen, C.A., and Andrade, L.E. Report of the First International Consensus on Standardized Nomenclature of Antinuclear Antibody HEp-2 Cell Patterns 2014-2015. Front Immunol. 6:412. Review.

Chan, E.K., de Melo Cruvinel, W., and Andrade, L.E. The International Consensus on Standardized Nomenclature of Antinuclear Antibody HEp-2 Cell Patterns (ICAP) initiative – Current state and perspectives. In: K. Conrad, E.K.L. Chan, L.E.C. Andrade, G. Steiner, G.J.M. Pruijn, Y. Shoenfeld (eds), From Autoantibody Research to Standardized Diagnostic Assays in the Management of Human Diseases series – Report on the 12th Dresden Symposium on Autoantibodies, Germany, p282-288.

Satoh, M., Tanaka, S., and Chan, E.K. Current status and issues of concern for the diagnostic autoantibody tests. In: K. Conrad, E.K.L. Chan, L.E.C. Andrade, G. Steiner, G.J.M. Pruijn, Y. Shoenfeld (eds), From Autoantibody Research to Standardized Diagnostic Assays in the Management of Human Diseases series – Report on the 12th Dresden Symposium on Autoantibodies, Germany, p298-306.

Dinse, G.E., Jusko, T.A., Whitt, I.Z., Co, C.A., Parks, C.G., Satoh, M., Chan, E.K., Rose, K.M., Walker, N.J., Birnbaum, L.S., Zeldin, D.C., Weinberg, C.R., and Miller, F.W. Associations between Selected Xenobiotics and Antinuclear Antibodies in the National Health and Nutrition Examination Survey, 1999-2004. Environ Health Perspect. [Epub ahead of print]

Satoh, M., Tanaka, S., and Chan, E.K. The uses and misuses of multiplex autoantibody assays in systemic autoimmune rheumatic diseases. Front Immunol. 6:181.

Fredenburg, K.M. and Chan, E.K. Our journey from the study of human autoantibodies to the microRNA world. Front Immunol. 6:110. Review.

Calise, S.J., Keppeke, G.D., Andrade, L.E., and Chan, E.K. Anti-rods/rings: a human model of drug-induced autoantibody generation. Front Immunol. 6:41. Review.

2014

Ceribelli, A., Fredi, M., Taraborelli, M., Cavazzana, I., Tincani, A., Selmi, C., Chan, J.Y., Chan, E.K., Satoh, M., and Franceschini, F. Prevalence and clinical significance of anti-MDA5 antibodies in European patients with polymyositis/dermatomyositis. Clin Exp Rheumatol. 32:891-7.

Parks, C.G., Miller, F.W., Satoh, M., Chan, E.K., Andrushchenko, Z., Birnbaum, L.S., Jusko, T.A., Kissling, G.E., Patel, M.D., Rose, K.M., Weinberg, C., Zeldin, D.C., and Sandler, D.P. Reproductive and hormonal risk factors for antinuclear antibodies (ANA) in a representative sample of U.S. women. Cancer Epidemiol Biomarkers Prev. 23:2492-502.

Mahler, M., Satoh, M., Hudson, M., Baron, M., Chan, J.Y., Chan, E.K., Wick, J., Fritzler, M.J., and the Canadian Scleroderma Research Group. Autoantibodies to the Rpp25 component of the Th/To complex are the most common antibodies in patients with systemic sclerosis without antibodies detectable by widely available commercial tests. J Rheumatol. 41:1334-43.

Keppeke, G.D., Satoh, M., Ferraz, M.L., Chan, E.K., and Andrade, L.E. Temporal evolution of human autoantibody response to cytoplasmic rods and rings structure during anti-HCV therapy with ribavirin and interferon-α. Immunol Res. 60:38-49.

Satoh, M., Chan, J.Y., Ceribelli, A., and Chan, E.K. Autoantibodies to survival of motor neuron (SMN) complex. In Autoantibodies (Shoenfeld, Y., Meroni, P.L., Gershwin, M.E., Eds.), pp. 139-144. Elsevier Science, Third Edition.

Satoh, M., Fritzler, M.J. and Chan, E.K. Anti-U1RNP and -Sm antibodies. In Autoantibodies (Shoenfeld, Y., Meroni, P.L., Gershwin, M.E., Eds.), pp. 151-159. Elsevier Science, Third Edition.

Fritzler, M.J. and Chan, E.K. Autoantibodies to GW/P bodies and components of the microRNA pathway. In Autoantibodies (Shoenfeld, Y., Meroni, P.L., Gershwin, M.E., Eds.), pp. 257-263. Elsevier Science, Third Edition.

Fritzler, M.J. and Chan, E.K. Golgi complex and endosome antibodies. In Autoantibodies (Shoenfeld, Y., Meroni, P.L., Gershwin, M.E., Eds.), pp. 265-273. Elsevier Science, Third Edition.

Ceribelli, A., Satoh, M., and Chan, E.K. Antinuclear Antibodies: General Introduction. In Autoantibodies, (Shoenfeld, Y., Meroni, P.L., Gershwin, M.E., Eds.), pp. 129-137. Elsevier Science, Third Edition.

Calise, S.J., Carcamo, W.C., Ceribelli, A., Dominguez, Y., Satoh, M., and Chan, E.K. Antibodies to rods and rings. In Autoantibodies (Shoenfeld, Y., Meroni, P.L., Gershwin, M.E., Eds.), pp. 161-168. Elsevier Science, Third Edition.

Carcamo, W.C., Calise, S.J., von Mühlen, C.A., Satoh, M., and Chan, E.K. Molecular cell biology and immunobiology of mammalian rod/ring structures. Int Rev Cell Mol Biol. 308:35-74. Review.

Agmon-Levin, N., Damoiseaux, J., Kallenberg, C., Sack, U., Witte, T., Herold, M., Bossuyt,  X., Musset, L., Cervera, R., Plaza-Lopez, A., Dias, C., Sousa, M.J., Radice, A., Eriksson, C., Hultgren, O., Viander, M., Khamashta, M., Regenass, S., Andrade, L.E., Wiik, A., Tincani, A., Rönnelid, J., Bloch, D.B., Fritzler, M.J., Chan, E.K., Garcia De La Torre, I., Konstantinov, K.N., Lahita, R., Wilson, M., Vainio, O., Fabien, N., Sinico, R.A., Meroni, P., and Shoenfeld, Y. International recommendations for the assessment of autoantibodies to cellular antigens referred to as anti-nuclear antibodies. Ann Rheum Dis. 73:17-23.

2013

Meroni, P.L., Chan, E.K., Tincani, A., Garcia de la Torre, I., and Andrade, L.E. Antinuclear antibody test: when to order? Am J Med. 126:e17.

Mahler, M., Gascon, C., Patel, S., Ceribelli, A., Fritzler, M.J., Swart, A., Chan, E.K., and Satoh, M. Rpp25 is a major target of autoantibodies to the Th/To complex as measured by a novel chemiluminescent assay. Arthritis Res Ther. 15:R50.

Petri, M.H., Satoh, M., Martin-Marquez, B.T., Vargas-Ramírez, R., Jara, L.J., Saavedra, M.A., Cruz-Gonzalez, C., Andrade-Ortega, L., Vera-Lastra, O., Salazar-Páramo, M., Prieto-Parra, R.E., Gonzalez-Lopez, L., Gamez-Nava, J.I., Ramírez-Sánchez, H.U., Chan, J.Y., Ross, S.J., Chan, E.K., and Vázquez-Del Mercado, M. Implications in the difference of anti-Mi-2 and -p155/140 autoantibody prevalence in two dermatomyositis cohorts from Mexico City and Guadalajara. Arthritis Res Ther. 15:R48.

Carcamo, W.C., Ceribelli, A., Calise, S.J., Krueger, C., Liu, C., Daves, M., Villalta, D., Bizzaro, N., Satoh, M., and Chan, E.K. Differential reactivity to IMPDH2 by anti-rods/rings autoantibodies and unresponsiveness to pegylated interferon-alpha/ribavirin therapy in US and Italian HCV patients. J Clin Immunol. 33:420-6.

Chan, E.K., Yao, B., and Fritzler, M.J. Reflections on ten years of history of, and future prospects for, GW182 and GW/P body research. In Ten Years of Progress in GW/P Body Research, edited by E.K.L. Chan and M.J. Fritzler, Adv Exp Med Biol, 768:261-70.

Chan, E.K. and Fritzler, M.J. Introduction – The GW body story as an example of autoantibodies with significant impacts to molecular cell biology. In Ten Years of Progress in GW/P Body Research, edited by E.K.L. Chan and M.J. Fritzler, Adv Exp Med Biol, 768:1-4.

Fritzler, M.J. and Chan, E.K. The discovery of GW bodies. In Ten Years of Progress in GW/P Body Research, edited by E.K.L. Chan and M.J. Fritzler, Adv Exp Med Biol, 768:5-21.

Satoh, M., Chan, J.Y., Ceribelli, A., Vazquez del-Mercado, M., and Chan, E.K. Autoantibodies to Argonaute 2 (Su antigen). In Ten Years of Progress in GW/P Body Research, edited by E.K.L. Chan and M.J. Fritzler, Adv Exp Med Biol, 768:45-59.

2012

Satoh, M., Ceribelli, A., and Chan, E.K. Common pathways of autoimmune inflammatory myopathies and genetic neuromuscular disorders. Clin. Rev. Allergy Immunol. 42:16-25.

Keppeke, G.D., Nunes, E., Ferraz, M.L., Silva, E.A., Granato, C., Chan, E.K., and Andrade, L.E. Longitudinal Study of a Human Drug-Induced Model of Autoantibody to Cytoplasmic Rods/Rings following HCV Therapy with Ribavirin and Interferon-α. PLoS One 7:e45392.

Ceribelli, A., Satoh, M., and Chan, E.K. A new immunoprecipitation-real time quantitative PCR assay for anti-Th/To and anti-U3RNP antibody detection in systemic sclerosis. Arthritis Res Ther. 14:R128.

Ceribelli, A., Fredi, M., Taraborelli, M., Cavazzana, I., Franceschini, F., Quinzanini, M., Tincani, A., Ross, S.J., Chan, J.Y., Pauley, B.A., Chan, E.K., and Satoh, M. Anti-MJ/NXP-2 autoantibody specificity in a cohort of adult Italian patients with polymyositis/dermatomyositis. Arthritis Res Ther. 14:R97.

Satoh, M., Chan, J.Y., Ross, S.J., Li, Y., Yamasaki, Y., Yamada, H., Vazquez-Del Mercado, M., Petri, M., Jara, L.J., Saavedra, M.A., Cruz-Reyes, C., Sobel, E.S., Reeves, W.H., Ceribelli, A., and Chan, E.K. Autoantibodies to transcription intermediary factor (TIF)1beta associated with dermatomyositis. Arthritis Res Ther. 14:R79.

Satoh, M., Ceribelli, A., and Chan, E.K. Role of environmental factors in autoantibody production – importance of a detailed analysis in a small cohort. Arthritis Res Ther. 14:109.

Satoh, M., Chan, E.K., Ho, L.A., Rose, K.M., Parks, C.G., Cohn, R.D., Jusko, T.A., Walker, N.J., Germolec, D.R., Whitt, I.Z., Crockett, P.W., Pauley, B.A., Chan, J.Y., Ross, S.J., Birnbaum, L.S., Zeldin, D.C., Miller, F.W. Prevalence and sociodemographic correlates of antinuclear antibodies in the United States. Arthritis Rheum. 64:2319-27.

Covini, G., Carcamo, W.C., Bredi, E., von Mühlen, C.A., Colombo, M., and Chan, E.K. Cytoplasmic rods and rings autoantibodies developed during pegylated interferon and ribavirin therapy in patients with chronic hepatitis C. Antivir Ther. 17:805-11.

2011

Carcamo, W.C., Satoh, M., Kasahara, H., Terada, N., Hamazaki, T., Chan, J.Y., Yao, B., Tamayo, S., Covini, G., von Mühlen, C.A., and Chan, E.K. Induction of cytoplasmic rods and rings structures by inhibition of the CTP and GTP synthetic pathway in mammalian cells. PLoS One. 6:e29690.

Satoh, M., Vázquez del Mercado, M., Krzyszczak, M.E., Li, Y., Ceribelli, A., Burlingame, R.W., Webb, T.T., Sobel, E.S., Reeves, W.H., and Chan, E.K. Coexistence of anti-RNA polymerase III and anti-U1RNP antibodies in patients with SLE – Two cases without features of scleroderma. Lupus 21:68-74.

Krzyszczak, M.E., Li, Y., Ross, S.J., Ceribelli, A., Chan, E.K., Bubb, M.R., Sobel, E.S., Reeves, W.H., and Satoh, M. Gender and ethnicity differences in the prevalence of scleroderma-related autoantibodies. Clin. Rheumatol. 30:1333-9.

Ceribelli, A., Krzyszczak, M.E., Li, Y., Ross, S.J., Chan, J.Y., Chan, E.K., Burlingame, R., Webb, T.T., Bubb, M.R., Sobel, E.S., Reeves, W.H., and Satoh, M. Atypical clinical presentation of a subset of patients with anti-RNA polymerase III – non-scleroderma cases associated with dominant RNA polymerase I reactivity and nucleolar staining. Arthritis Res. Ther. 13:R119.

Satoh, M., Krzyszczak, M.E., Li, Y., Ceribelli, A., Ross, S.J., Chan, E.K., Segal, M.S., Bubb, M.R., Sobel, E.S., and Reeves, W.H. Frequent coexistence of anti-topoisomerase I and anti-U1RNP autoantibodies in African American patients associated with mild skin involvement: a retrospective clinical study. Arthritis Res. Ther. 13:R73.

Satoh, M., Chan, J.Y., Ross, S.J., Ceribelli, A., Cavazzana, I., Franceschini, F., Li, Y., Reeves, W.H., Sobel, E.S., and Chan, E.K. Autoantibodies to survival of motor neuron (SMN) complex in patients with polymyositis – Immunoprecipitation of D-E-F-G without other components of small nuclear ribonucleoproteins. Arthritis Rheum. 63:1972-8.

Satoh, M., Bjerkås, I., Haugarvoll, E., Chan, E.K., Szabo, N.J., Jirillo, E., Poppe, T.T., Sveier, H., Tørud, B., and Koppang, E.O. Polyclonal hypergammaglobulinemia and autoantibody production induced by vaccination in farmed Atlantic salmon. Fish Shellfish Immunol. 30:1080-6.

Ceribelli, A., Tincani, A., Cavazzana, I., Franceschini, F., Cattaneo, R., Pauley, B.A., Chan, J.Y., Chan, E.K., and Satoh, M. Anti-argonaute2 (Ago2/Su) and -Ro antibodies identified by immunoprecipitation in primary anti-phospholipid syndrome (PAPS). Autoimmunity 44:90-7.

2010

Vázquez-Del Mercado, M., Sánchez Orozco, L.V., Pauley, B.A., Chan, J.Y., Chan, E.K., Panduro, A., Maldonado González, M., Jimenez-Luevanos, M.A., Martin Márquez, B.T., Palafox-Sanchez, C.A., Dávalos-Rodríguez, I.P., Salazar-Páramo, M., González Lopez, L., Gámez Nava, J.I., and Satoh, M. Autoantibodies to a miRNA-binding protein Argonaute 2 (Su antigen) in patients with hepatitis C virus infection. Clin. Exp. Rheumatol. 28:842-8.

Ceribelli, A., Cavazzana, I., Franceschini, F., Airò, P., Tincani, A., Cattaneo, R., Pauley, B.A., Chan, E.K., and Satoh, M. Anti-Th/To are common anti-nucleolar autoantibodies in Italian scleroderma patients. J. Rheumatol. 37:2071-5.

Pereira, D.B., Sannes, T., Dodd, S.M., Jensen, S.E., Morgan, L.S., and Chan, E.K. Life stress, negative mood states, and antibodies to heat shock protein 70 in endometrial cancer. Brain, Behavior, and Immunity 24:210-4.

Vázquez-Del Mercado, M., Palafox-Sánchez, C.A., Muñoz-Valle, J.F., Orozco-Barocio, G., Oregon Romero, E., Navarro Hernández, R.E., Salazar-Páramo, M., Chan, J.Y., Chan, E.K., and Satoh, M. High prevalence of autoantibodies to RNA helicase A in Mexican patients with systemic lupus erythematosus. Arthritis Res. Ther. 12:R6.

Chan, E.K. Autoantibodies to multiple components of the microRNA pathway and aberrant microRNA expression in autoimmune diseases. In: Jack D. Keene (ed), Autoantibodies and Autoimmunity, MBL, Tokyo, Japan, pp45-59.

Satoh, M., Fritzler, M.J., and Chan, E.K. Anti-histone and anti-spliceosomal antibodies. In Systemic Lupus Erythematosus, 5th ed. edited by Robert G. Lahita. Academic Press, San Diego, Chapter 16, pp. 275- 292.

2009

Satoh, M., Vázquez-Del Mercado, M., and Chan, E.K. Clinical interpretation of antinuclear antibody tests in systemic rheumatic diseases. Mod. Rheum. 19:219-28.

Kakumanu, P., Sobel, E.S., Narain, S., Li, Y., Akaogi, J., Yamasaki, Y., Segal, M.S., Hahn, P.C., Chan, E.K., Reeves, W.H., and Satoh, M. Citrulline dependence of anti-CCP antibodies in SLE as a marker of deforming/erosive arthritis. J. Rheumatol. 36:2682-90.

Brucato, A., Grava, C., Bortolati, M., Ikeda, K., Milanesi, O., Cimaz, R., Ramoni, V., Vignati, G., Martinelli, S., Sadou, Y., Borghi, A., Tincani, A., Chan, E.K., and Ruffatti, A. Congenital Heart Block not associated with anti-Ro/La antibodies: comparison with anti-Ro/La-positive cases. J. Rheumatol. 36:1744-8.

Nozawa, K., Ikeda, K., Satoh, M., Reeves, W.H., Stewart, C., Li, Y.C., Yen, T.J., Rios, R.M., Takamori, K., Ogawa, H., Sekigawa, I., Takasaki, Y., and Chan, E.K. Autoantibody to NA14 is an independent marker primarily for Sjögren’s syndrome. Frontiers Biosci. 14:3733-9.

Palafox-Sánchez, C.A., Satoh, M., Chan, E.K., Carcamo, W.C., Muñoz-Valle, J.F., Orozco-Barocio, G., Oregón-Romero, E., Navarro-Hernández, R.E., Salazar-Páramo, M., Castañeda, A.C., and Vázquez-Del Mercado, M. Reduced IgG anti-snRNPs autoantibody production in SLE patients with positive IgM anti-cytomegalovirus antibodies. Arthritis Res. Ther. 11:R27.

Llanos, C., Chan, E.K., Li, S., Abadal, G.X., Izmirly, P., Byrne, C., Clancy, R.M., and Buyon, J.P. Antibody Reactivity to α-Enolase in Mothers of Children with Congenital Heart Block. J. Rheumatol. 36:565-9.

2008

Koppang, E.O., Bjerkås, I., Haugarvoll, E., Chan, E.K., Szabo, N.J., Ono, N., Akikusa, B., Jirillo, E., Poppe, T.T., Sveier, H., Tørud, B., and Satoh, M. Vaccination-induced systemic autoimmunity in farmed Atlantic salmon. J. Immunol. 181:4807-14.

Nozawa, K., Fritzler, M.J., Ikeda, K., Takasaki, Y., Satoh, M., and Chan, E.K. Differential anti-Golgi complex autoantibody production in murine lactate dehydrogenase-elevating virus infection. Immunopharmacology and Immunotoxicology 30:13-25.

Kakumanu, P., Yamagata, H., Sobel, E.S., Reeves, W.H., Chan, E.K., and Satoh, M. Patients with pulmonary tuberculosis are frequently positive for anti-CCP antibodies but they also react with unmodified arginine peptide. Arthritis Rheum. 58:1576-81.

2007

Satoh, M., Chan, E.K., Sobel, E.S., Kimpel, D.L., Yamasaki, Y., Narain, S., Mansoor, R., and Reeves, W.H. Clinical implication of autoantibodies in patients with systemic rheumatic diseases. Expert Rev. Clin. Immunol. 3:721-38.

Chan, E.K., Fritzler, M.J., Wiik, A., Andrade, L.E.C., Reeves, W.H., Tincani, A., Meroni, P.L., and the IUIS/WHO/AF/CDC committee for the standardization of autoantibodies in rheumatic and related diseases. AutoAbSC.Org – Autoantibody Standardization Committee in 2006. Autoimmunity Rev. 6:577-80.

Bhanji, R.A., Eystathioy, T., Chan, E.K., Bloch, D.B., and Fritzler, M.J. Clinical and serological features of patients with autoantibodies to GW/P Bodies. Clin. Immunol. 125:247-56.

Yamasaki, Y., Narain, S., Yoshida, H., Hernandez, L., Barker, T., Hahn, P.C., Sobel, E.S., Segal, M.S., Richards, H.B., Chan, E.K., Reeves, W.H., and Satoh, M. Autoantibodies to RNA helicase A: A new serological marker of early lupus. Arthritis Rheum. 56:596-604.

Zhang, J.Y., Megliorino, R., Peng, X.X., Tan, E.M., Chen, Y., and Chan, E.K. Antibody detection using tumor-associated antigen mini-array in immunodiagnosing human hepatocellular carcinoma. J. Hepatol. 46:107-114.

2006

Yamasaki, Y., Yamada, H., Nozaki, T., Akaogi, J., Nichols, C., Lyons, R., Loy, A.C., Chan, E.K., Reeves, W.H., and Satoh, M. Unusually high frequency of autoantibodies to PL-7 associated with milder muscle disease in Japanese patients with polymyositis/dermatomyositis. Arthritis Rheum. 54:2004-9.

Yamasaki, Y., Narain, S., Hernandez, L., Barker, T., Ikeda, K., Segal, M.S., Richards, H.B., Chan, E.K., Reeves, W.H., and Satoh, M. Autoantibodies against the replication protein A complex in SLE and other autoimmune diseases. Arthritis Res. Ther. 8:R111.

Yamasaki, Y., Narain, S., Ikeda, K., Honkannen-Scott, M., Hernandez, B.S., Barker, T., Richards, H.B., Chan, E.K., Reeves, W.H., and Satoh, M. Nucleolar staining cannot be used as a screening test for the scleroderma marker anti-RNA polymerase I/III antibodies. Arthritis Rheum. 54:3051-6.

Jakymiw, A., Ikeda, K., Fritzler, M.J., Reeves, W.H., Satoh, M., and Chan, E.K. Autoimmune targeting of key components of RNA interference. Arthritis Res. Ther. 8:R87.

2005

Lu, Y., Ye, P., Chen, S-L., Tan, E.M., and Chan, E.K. Identification of kinectin as a novel Behçet’s disease autoantigen. Arthritis Res. Ther. 7:R1133-9.

Clancy, R.M., Buyon, J.P., Ikeda, K., Nozawa, K., Argyle, D.A., Friedman, D.M., and Chan, E.K. Maternal antibody responses to the 52kD SS-A/Ro p200 peptide and the development of fetal conduction defects. Arthritis Rheum. 52:3079-86.

2004

Nozawa, K., Fritzler, M.J., von Mühlen, C.A., and Chan, E.K. Giantin is the major Golgi autoantigen in human anti-Golgi complex sera. Arthritis Res. Ther. 6:R95-R102.

2003

Eystathioy, T., Chan, E.K., Takeuchi, K., Mahler, M., Luft, L.M., Zochodne, D.W., and Fritzler, M.J. Clinical and serological associations of autoantibodies to GW bodies and a novel cytoplasmic autoantigen GW182. J. Mol. Med., 81:811-818.

2002

Eystathioy, T., Chan, E.K., Tenenbaum, S.A., Keene, J.D., Griffith, K., and Fritzler, M.J. A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles. Mol. Biol. Cell 13: 1338-51.